The antibodies 14C2 and Z3 were originally isolated as IgGs and recognize the ecto domain of the M2 influenza protein. The antibody named p1-1, was isolated as an scFv in an in vitro selection campaign recognizes the phosphorylated ITAM peptide of FceR1 receptor of the allergy pathway. a) Yeast display plot for the three antibodies in both scFv and scTGP formats. The x-axis shows the expression level measured using PE labeled anti-SV5 antibody, which recognizes the SV5 expression tag in the vector system. The y-axis depicts the antigen recognition signal. Biotinylated M2 peptide was the specific antigen for the 14C2 and Z3 and biotinylated ITAM peptide p1-1 was the antigen used to characterize p1-1. The biotinylated peptides were labeled wi...
Phage display is a powerful technology for selection of novel binding functions from large populatio...
<p>A: Western Blot analysis showing molecular size of scFv-Fc fusion antibodies B6-11, B6-112 and B6...
Additional file 1: Figure S1. Construction of scFv antibody gene. (a) The amino acid sequences of va...
Phage display technology is one of the methods widely adopted for the production of human monoclonal...
Novel methods are reported for evaluating and utilizing single chain fragment variable (scFv) antibo...
a) data on E.coli based protein expression from various scTGP clones. The fluorescence signal obtain...
a) the top row compares the display levels and antigen recognition of the parental scFv libraries an...
Using a cell-bound immunogen, we have generated a monoclonal antibody, 3D5, that recognizes carboxy-...
<p><b>b</b>. 16% SDS-PAGE analysis of F1 protein expression [<b>A</b>]. 12% SDS–PAGE analysis of Lcr...
<p>After transferring the scFv coding region from the phagemid into an expression vector, these five...
Abstract Background The influenza virus enters the host via hemagglutinin protein binding to cell su...
<p><b>Copyright information:</b></p><p>Taken from "Generation of human scFvs antibodies recognizing ...
<p><b>Copyright information:</b></p><p>Taken from "Generation of human scFvs antibodies recognizing ...
The selected scFv library is amplified by inverse PCR and CPEC assembly is used to build the scFP li...
For ease of detection, soluble forms of phage-displayed scFv antibodies are usually expressed with a...
Phage display is a powerful technology for selection of novel binding functions from large populatio...
<p>A: Western Blot analysis showing molecular size of scFv-Fc fusion antibodies B6-11, B6-112 and B6...
Additional file 1: Figure S1. Construction of scFv antibody gene. (a) The amino acid sequences of va...
Phage display technology is one of the methods widely adopted for the production of human monoclonal...
Novel methods are reported for evaluating and utilizing single chain fragment variable (scFv) antibo...
a) data on E.coli based protein expression from various scTGP clones. The fluorescence signal obtain...
a) the top row compares the display levels and antigen recognition of the parental scFv libraries an...
Using a cell-bound immunogen, we have generated a monoclonal antibody, 3D5, that recognizes carboxy-...
<p><b>b</b>. 16% SDS-PAGE analysis of F1 protein expression [<b>A</b>]. 12% SDS–PAGE analysis of Lcr...
<p>After transferring the scFv coding region from the phagemid into an expression vector, these five...
Abstract Background The influenza virus enters the host via hemagglutinin protein binding to cell su...
<p><b>Copyright information:</b></p><p>Taken from "Generation of human scFvs antibodies recognizing ...
<p><b>Copyright information:</b></p><p>Taken from "Generation of human scFvs antibodies recognizing ...
The selected scFv library is amplified by inverse PCR and CPEC assembly is used to build the scFP li...
For ease of detection, soluble forms of phage-displayed scFv antibodies are usually expressed with a...
Phage display is a powerful technology for selection of novel binding functions from large populatio...
<p>A: Western Blot analysis showing molecular size of scFv-Fc fusion antibodies B6-11, B6-112 and B6...
Additional file 1: Figure S1. Construction of scFv antibody gene. (a) The amino acid sequences of va...