SARS-CoV-2 inactivation and cellular fixation.
- Raven M. Osborn (14676015)
- Justin Leach (14676018)
- Michelle Zanche (14676021)
- John M. Ashton (12400146)
- ChinYi Chu (14676024)
- Juilee Thakar (31567)
- Stephen Dewhurst (11665)
- Sonia Rosenberger (14676027)
- Martin Pavelka (453851)
- Gloria S. Pryhuber (10014764)
- Thomas J. Mariani (8998040)
- Christopher S. Anderson (3141414)
Publication date
February 2023
DOI Publisher
Public Library of Science (PLoS)Abstract
VeroE6 cells were infected with SARS-CoV-2 for 96 hrs and fixed with methanol-acetone. They were then examined for the presence of SARS-CoV-2-induced cytopathic effects. (A), infectious virus (B) and cell viability (C). (A) Representative images of cytopathic effects of SARS-CoV-2 at 96 hrs post infection at 10X magnification. (B) Bar plot of TCID50 results. Time indicates the length of methanol-acetone fixation. (C) Density plot showing the kernel density estimation of flow cytometry results from uninfected cells using the cell viability dye, calcein green. M-A indicates methanol-acetone fixation. *indicates unstained control.</p
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