S7 Fig -
- Vitoria Isabela Montanhero Cabrera (14676145)
- Gabrielle do Nascimento Sividanes (14676148)
- Natalia Fernanda Quintiliano (14676151)
- Marcos Hikari Toyama (4911514)
- João Henrique Ghilardi Lago (11880391)
- Marcos Antonio de Oliveira (9201605)
Publication date
February 2023
DOI Publisher
Public Library of Science (PLoS)Abstract
Purification by IMAC of recombinant enzymes PaAhpC (A), SeAhpC (B), EcTrx (C), EcTrxR (D), HsPrx2 (E), ScTrx1 (F) and ScTrxR (G) expressed in E. coli BL21 (DE3). Reducing SDS-PAGE (200mM—mercaptoethanol, 12% polyacrylamide) representative of purification results of recombinant His-tag-containing proteins purified by IMAC (A, B, C, D, E, F, G) (using HiTrap TALON crude column (Cytiva Life Sciences) or by the boiling method (F). The black arrow denotes the position of the recombinant protein in the gel. (TIFF)</p
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