Sphingomyelin levels in urinary extracts and those in tissues from the kidneys of standard diet (STD)- and high-fat diet (HFD)-fed mice in the phlorizin assay.

Liquid chromatography-tandem mass spectrometry was used to quantify sphingomyelin levels in urinary extracts from (A) total sphingomyelin (d18:0), sphingomyelin (d18:1), (B) total ceramide (d18:0), and ceramide (d18:1) in the HFD obese mouse model in the phlorizin assay. (C) Total sphingomyelin (d18:0), sphingomyelin (d18:1), (D) total ceramide (d18:0), and ceramide (d18:1) of tissues in the HFD obese mouse model from the phlorizin assay. Results are presented as the mean ± standard deviation. *P P < 0.01. Statistical significances were calculated by two-way analysis of variance followed by the post-hoc Turkey–Kramer tests and Sidak’s multiple comparison test. Urine was obtained from C57/BL6J mice fed with a STD (STD-Fed) or HFD (HFD-Fed) prior to vehicle administration, vehicle-treated mice (Veh) prior to phlorizin or vehicle administration, phlorizin- or vehicle-treated mice on day one (PLZ-D1 or Veh-D1), and phlorizin- or vehicle-treated mice on day two (PLZ-D2 or Veh-D2).