A specific TRPV1 antagonist, BCTC, overrides the effects of capsaicin in TRPV1-overexpressing HEK293T cells.

Dose-response curve of the TRPV1 antagonist BCTC against capsaicin in human TRPV1-transfected HEK293T cells. Left panel: time courses of normalized fluorescence displayed as mean of two individual runs (range in grey) recorded with a FlexStation3. BCTC (1 to 1000 nmol/L) was added automatically at 20 s, capsaicin 0.5 μmol/L at 60 s. Right panel: dose response curve, given by area under the curve of fluorescence intensity over the capsaicin 0.5 μmol/L exposure. Two independent runs, represented as mean with range, were fitted with a sigmoidal function. (TIF)