Fig 4 -

(A) Sagittal sections of representative osteotomized and lengthened tibiae of wild-type (WT) (left column) and Cnmd-/- (right column) mice at 1, 2, and 3 weeks after osteotomy, stained with toluidine blue. Areas showing metachromasia represent cartilage callus. Scale bar, 500 μm. (B) The callus areas of the cartilage in both genotypes at 1, 2, and 3 weeks after osteotomy were measured by histomorphometry. Data are shown as mean ± SE. (C) Sox9, Col2a1 and Col10a1 mRNA expression levels at 1, 2, and 3 weeks after osteotomy were measured by real-time RT-PCR, and were normalized to Gapdh was calculated. Data are shown as mean ± SE. Statistic examination was performed using ANOVA and significant differences are indicated by symbols: *p Cnmd-/- mice.