Features of pea centromere 6 (CEN6).

(A) Immunolabeling of CENH3 protein (green) on metaphase chromosome 6 (counterstained with DAPI, gray). (B) Position of the primary constriction in the assembly. Arrows below the scale indicate the 5.2 Mb tandem duplication, and the arrowhead shows the position of a single gap in the assembly. (C) Distribution of CENH3 chromatin revealed by ChIP-seq experiments using anti-CENH3-1 and anti-CENH3-2 antibodies. Peaks in the graphs correspond to the statistically significant enrichment ratio of ChIP reads to control input reads (see S2 Fig for full data analysis including experimental replicates). (D) Positions of large arrays of satellite repeats. Different repeat families are marked by different colors, as indicated in the legend. (E) Densities of different types of repetitive DNA sequences and predicted genes calculated in 100 kb windows. (F) Cytosine methylation profiles calculated as the ratio of methylated cytosines to all cytosines present in the sequence. Ratios were calculated separately for cytosines in three different contexts (distinguished by plot colors) and averaged for 100 kb windows.