Genome wide effects of Rho over-production on the <i>B</i>. <i>subtilis</i> transcriptome during exponential growth and stationary phase in rich medium.

Scatter plots display the transcriptome changes in the antisense (A and B) and sense (C and D) strands by comparing B. subtilis Rho+ and WT strains during exponential growth (A and C) and stationary phase (B and D), respectively. Horizontal and vertical dashed lines correspond to the cut-off for minimal expression at log2(fpkm+5)≥5, as in Fig 6. The diagonal median solid line indicates unchanged expression levels, while dashed and dotted lines delineate DE changes of the |log2FC|≥1 and |log2FC|≥2, respectively. Each point represents one of the 4,292 AL009126.3-annotated genes. Point coordinates on x- and y-axes correspond to the normalized expression level (average of log2(fpkm+5) over biological replicates) measured with RNAseq in B. subtilis WT and Rho+, respectively. Background colors of the points indicate TRs whose transcription level is strongly up-regulated (yellow) or down-regulated (blue) in the Rho+ vs. WT comparison. (TIF)