The work presented here describes the development of a single integrated microfluidic device for RNA extraction using silica-based monoliths and the amplification of mRNA using reverse transcription-polymerase chain reaction (RTPCR). PCR products are detected in real-time using PicoGreen™, a fluorescent dye that intercalates into double stranded (ds) DNA that can be quantified using a laserbased detection system
Nucleic acid amplification is a key step in nucleic acid detection assays. The use of digital microf...
Droplet microfluidics can identify and sort cells using digital reverse transcription polymerase cha...
<p>(A) Drops containing RNA and RT-PCR reagents are created with a microfluidic T-junction and carri...
The first lab-on-chip system for picoliter droplet generation and RNA isolation, followed by reverse...
This paper describes the development of a microfluidic methodology, using RNA extraction and reverse...
This presentation reports an integrated DNA extraction and amplification device based on electro-osm...
A micro-fabricated Polymerase chain reaction (PCR) device with integrated temperature cycling and fl...
The development of microfluidic tools for high-throughput nucleic acid analysis has become a burgeon...
Integrated DNA extraction and amplification have been carried out in a microfluidic device using ele...
In this Study, a new polymerase chain reaction (PCR) system incorporated with a fluorescence detecti...
abstract: A major goal of the Center for Biosignatures Discovery Automation (CBDA) is to design a di...
ABSTRACT Real-time quantitative Polymerase Chain Reaction (PCR) is an extremely sensitive and reliab...
Real-time, fluorescence-based reverse transcription poly-merase chain reaction (RT-PCR)[1] has been ...
Microfluidic techniques have allowed for fast, sensitive, and low cost applications of the Polymeras...
Quantitative, reverse transcription, polymerase chain reaction (qRT-PCR) is facilitated by leveragin...
Nucleic acid amplification is a key step in nucleic acid detection assays. The use of digital microf...
Droplet microfluidics can identify and sort cells using digital reverse transcription polymerase cha...
<p>(A) Drops containing RNA and RT-PCR reagents are created with a microfluidic T-junction and carri...
The first lab-on-chip system for picoliter droplet generation and RNA isolation, followed by reverse...
This paper describes the development of a microfluidic methodology, using RNA extraction and reverse...
This presentation reports an integrated DNA extraction and amplification device based on electro-osm...
A micro-fabricated Polymerase chain reaction (PCR) device with integrated temperature cycling and fl...
The development of microfluidic tools for high-throughput nucleic acid analysis has become a burgeon...
Integrated DNA extraction and amplification have been carried out in a microfluidic device using ele...
In this Study, a new polymerase chain reaction (PCR) system incorporated with a fluorescence detecti...
abstract: A major goal of the Center for Biosignatures Discovery Automation (CBDA) is to design a di...
ABSTRACT Real-time quantitative Polymerase Chain Reaction (PCR) is an extremely sensitive and reliab...
Real-time, fluorescence-based reverse transcription poly-merase chain reaction (RT-PCR)[1] has been ...
Microfluidic techniques have allowed for fast, sensitive, and low cost applications of the Polymeras...
Quantitative, reverse transcription, polymerase chain reaction (qRT-PCR) is facilitated by leveragin...
Nucleic acid amplification is a key step in nucleic acid detection assays. The use of digital microf...
Droplet microfluidics can identify and sort cells using digital reverse transcription polymerase cha...
<p>(A) Drops containing RNA and RT-PCR reagents are created with a microfluidic T-junction and carri...