Ct-values of strand specific RT-qPCR for detection of vRNA, cRNA and mRNA for NA (D-F) or NP (G-I) of indicated H5N1 viruses in MDCK-II cells infected with an MOI of 1 for 4, 8, 12 and 24 h. Results are shown as mean and standard deviation of two triplicate experiments. Asterisks indicate significant differences at p < 0.05.</p
Influenza A virus is a negative-strand RNA virus with a genome comprising of eight individual segmen...
<p>Cells infected with NL4-3 virus were harvested 6 hours post infection. Nuclear and cytoplasmic fr...
<p>RNA from 12 probands, extracted with 6 RNA isolation kits from two collection tubes (PAXgene [P] ...
<p>(A) Quantification of vRNA and mRNA in infected cells. Six hours after MDCK infection with rescue...
<p>Six hours after MDCK infection with rescued influenza A1 (black bars) or A8 (white bars) viruses ...
A549 cells were infected with the viruses at MOI of 2 and viral mRNA (A) and vRNA (B) were quantitat...
(A) NA activity in cell lysates and supernatants at different time points following infection. Cells...
Model fit (lines) to experimental data (circles ± standard deviation, n = 4 or single circles for FA...
<p>Virus antigens were distributed within the cytoplasm and the nucleus of the infected cells (<b>A-...
<p>(A) HAP1<sub>WT</sub> or HAP1<sub>S1P</sub> cells were infected with VSV-(G) or rVSV-ANDV for 12 ...
<p>A549 cells were infected with A/Beijing/353/89 (H3N2), A/Vietnam/1194/04 (H5N1) and A/Anhui/1/13 ...
<p>(A) Brief protocol and incubation periods are indicated. (B) Quantitation of cRNA (closed circle)...
<p>A549, MDCK, and CEF cells were infected either with the H1N1/WSN (♦), H9N2 (▴), H5N2/F118 (▪) or ...
<p>293T cells were co-transfected with expression plasmids of NP, PA, PB1 and either wild type (WT) ...
<p>(a) HA RNA quantification in supernatant. RNA was quantified with a qPCR assay at 12, 24, 36, and...
Influenza A virus is a negative-strand RNA virus with a genome comprising of eight individual segmen...
<p>Cells infected with NL4-3 virus were harvested 6 hours post infection. Nuclear and cytoplasmic fr...
<p>RNA from 12 probands, extracted with 6 RNA isolation kits from two collection tubes (PAXgene [P] ...
<p>(A) Quantification of vRNA and mRNA in infected cells. Six hours after MDCK infection with rescue...
<p>Six hours after MDCK infection with rescued influenza A1 (black bars) or A8 (white bars) viruses ...
A549 cells were infected with the viruses at MOI of 2 and viral mRNA (A) and vRNA (B) were quantitat...
(A) NA activity in cell lysates and supernatants at different time points following infection. Cells...
Model fit (lines) to experimental data (circles ± standard deviation, n = 4 or single circles for FA...
<p>Virus antigens were distributed within the cytoplasm and the nucleus of the infected cells (<b>A-...
<p>(A) HAP1<sub>WT</sub> or HAP1<sub>S1P</sub> cells were infected with VSV-(G) or rVSV-ANDV for 12 ...
<p>A549 cells were infected with A/Beijing/353/89 (H3N2), A/Vietnam/1194/04 (H5N1) and A/Anhui/1/13 ...
<p>(A) Brief protocol and incubation periods are indicated. (B) Quantitation of cRNA (closed circle)...
<p>A549, MDCK, and CEF cells were infected either with the H1N1/WSN (♦), H9N2 (▴), H5N2/F118 (▪) or ...
<p>293T cells were co-transfected with expression plasmids of NP, PA, PB1 and either wild type (WT) ...
<p>(a) HA RNA quantification in supernatant. RNA was quantified with a qPCR assay at 12, 24, 36, and...
Influenza A virus is a negative-strand RNA virus with a genome comprising of eight individual segmen...
<p>Cells infected with NL4-3 virus were harvested 6 hours post infection. Nuclear and cytoplasmic fr...
<p>RNA from 12 probands, extracted with 6 RNA isolation kits from two collection tubes (PAXgene [P] ...