Meningeal and vascular inflammation is associated with neuronal infection at the choroid plexus.

(A, B) Immunohistochemistry of SARS-CoV-2 nucleocapsid, neuronal NeuN, and glial cell GFAP of the cerebral cortex adjacent to the choroid plexus 2 and 5–6 days after infection. Arrows indicated nucleocapsid staining. Red dotted lines trace ependymal cells of the choroid plexus. (C, D) HE staining of brain choroid plexus and cerebral cortex blood vessels 2 and 5–6 days after infection of hACE2fl/y and ShhCre/+; hACE2fl/y mice. Arrows indicate sites of immune cell infiltration. Representative of N = 4 animals per genotype and time point. Scale bars 50 μm. HE, hematoxylin–eosin; SARS-CoV-2, Severe Acute Respiratory Syndrome Coronavirus 2. (TIF)