A3s expression in WT- and modified- A549 and HBEC3-KT cells.

A549 cells were infected with HAdV-A12, -B3, -C2 or mock control at a MOI = 3 and analyzed 2 days post infection. HBEC3-KT were infected with HAdV-A12, -B3, -C2 or mock control at a MOI = 1 and analyzed 4 days post infection. A3s expression levels were also reported in uninfected A549 shA3B and HBEC3-KT A3B. (A) The A3 mRNAs were quantified by RT-qPCR and expressed relative the GAPDH. The A3A, A3D and A3H mRNAs were not detected. (B) The A3B protein levels were assessed by western blot using the 5210-87-13 mAb antibody, quantified by densitometry and normalized with HSP90. Panel B displays a full blot from 15kDa to 55 kDa. (TIFF)