Feeding behavior of <i>Myzus persicae</i> on healthy plants after acquisition of P2 and P3:virions in artificial medium.

The histogram bars display means and standard errors. Before recording aphid feeding behavior on healthy plants, aphids were allowed to feed under electrical penetration graph (EPG) control for 1 h on different artificial media: 15% sucrose in water (light grey); 15% sucrose in DB5 buffer (orange) or 15% sucrose (final) in DB5 buffer supplemented with P3 and purified virus particles (P3:virions, dark grey); his-tagged P2 (HP2, yellow); or HP2 and P3:virions (blue). Purity of the viral components used for aphid feeding assays on artificial medium is shown in S2 Fig. Only aphids having inserted their stylets for at least 5 min in the artificial media were used for the experiments (N = 21–26). Letters indicate significant differences between artificial media as tested by GLM (generalized linear model) followed by pairwise comparisons using “emmeans” (p 2 = 23.228, p 2 = 20.090, p 2 = 72.429; p 2 = 21.336, p 2 = 72.430, p < 0.001).