A3B reduces viral load, progression to late phase transcription, and production of infectious viral particles with variable efficacy between viral strains.

HBEC-WT, -GFP, -A3B and -A3B-DD were infected with HAdV-A12, -B3 or -C2 at a MOI = 3. (A) The levels of different viral mRNAs were quantified by RTqPCR after 12 and 24-hours post infection. An immediate early transcript (E1A), an early transcript (E4orf6) and a late transcript (Penton) were quantified. The penton mRNA could not be reliably detected at 12-hours post infection. (B) The levels of viral DNA were quantified by qPCR at 24 hours post infection. (C) The levels of infectious viral particles were quantified by foci forming assay at 24 hours post infection. P-values were calculated by ratio t-tests. Only significant differences are shown.