Measuring the stoichiometry of functional PspA complexes in living bacterial cells by single molecule photobleaching

A single-molecule method for measuring fluorophore labeling yields for the study of membrane protein oligomerization in membranes.

Melanie Ernst
Tugba N Ozturk
Janice L Robertson

January 2023

Membrane proteins are often observed as higher-order oligomers, and in some cases in multiple stoich...

Visualizing single molecular complexes in vivo using advanced fluorescence microscopy.

Dobbie, IM
Robson, A
Delalez, N
Leake, MC

January 2009

Full insight into the mechanisms of living cells can be achieved only by investigating the key proce...

Probing cellular protein complexes using single-molecule pull-down

Jain, Ankur

December 2013

Cellular processes result from dynamic interactions between biomolecules. The gold standard method f...

Measuring the stoichiometry of functional PspA complexes in living bacterial cells by single molecule photobleaching

Lenn, Tchern
Gkekas, Christos N.
Bernard, Laurent
Engl, Christoph
Jovanović, Goran
Buck, Martin
Ying, Liming

January 2011

We report a general method based on wide-field fluorescence imaging of single molecule photobleachin...

The development of a single molecule fluorescence standard and its application in estimating the stoichiometry of the nuclear pore complex

Tie, Hieng Chiong
Madugula, Viswanadh
Lu, Lei

January 2016

We report here an image-based method to quantify the stoichiometry of diffraction-limited sub-cellul...

The development of a single molecule fluorescence standard and its application in estimating the stoichiometry of the nuclear pore complex

Tie, Hieng Chiong
Madugula, Viswanadh
Lu, Lei

September 2016

AbstractWe report here an image-based method to quantify the stoichiometry of diffraction-limited su...

Stoichiometry of molecular complexes at adhesions in living cells.

Digman, Michelle A
Wiseman, Paul W
Choi, Colin
Horwitz, Alan R
Gratton, Enrico

February 2009

We describe a method to detect molecular complexes and measure their stoichiometry in living cells f...

Quantitative Brightness Analysis of Fluorescence Intensity Fluctuations in E. Coli.

Kwang-Ho Hur
Joachim D Mueller

The brightness measured by fluorescence fluctuation spectroscopy specifies the average stoichiometry...

Exploring fluorescence antibunching in solution to determine the stoichiometry of molecular complexes

Sykora, J.
Kaiser, K.
Gregor, I.
Bönigk, W.
Schmalzing, G.
Enderlein, J.

January 2007

Fluorescence antibunching is a well-known technique for determining the number of independent emitte...

Quantitative Brightness Analysis of Fluorescence Intensity Fluctuations in E. Coli

Kwang-Ho Hur (562936)
Joachim D. Mueller (562940)

June 2015

<div><p>The brightness measured by fluorescence fluctuation spectroscopy specifies the average stoic...

Single Cell GFP-Trap Reveals Stoichiometry and Dynamics of Cytosolic Protein Complexes

Tim Wedeking (1675264)
Sara Löchte (1675258)
Christian P. Richter (1675267)
Maniraj Bhagawati (1675270)
Jacob Piehler (348230)
Changjiang You (1675261)

May 2015

We developed in situ single cell pull-down (SiCPull) of GFP-tagged protein complexes based on microp...

Fluorescence resonance energy transfer-based stoichiometry in living cells

Kenneth Christensen
Joel A. Swanson

January 2002

ABSTRACT Imaging of fluorescence resonance energy transfer (FRET) between fluorescently labeled mole...

A general approach to break the concentration barrier in single-molecule imaging

Loveland, Anna B.
Habuchi, Satoshi
Walter, Johannes C.
Oijen, Antoine M. van

January 2012

Single-molecule fluorescence imaging is often incompatible with physiological protein concentrations...

Protocol for single-molecule fluorescence recovery after photobleaching microscopy to analyze the dynamics and spatial locations of nuclear transmembrane proteins in live cells

Mark Tingey
Yichen Li
Weidong Yang

June 2021

Summary: Single-molecule fluorescence recovery after photobleaching (smFRAP) is a newly developed te...

Single-molecule fluorescence of internalised biomolecules in live bacteria

Plochowietz, Anne
Kapanidis, Achillefs N

January 2012

In the last two decades emerging single-molecule fluorescence tools have been developed and adapted ...

A single-molecule method for measuring fluorophore labeling yields for the study of membrane protein oligomerization in membranes.

Melanie Ernst
Tugba N Ozturk
Janice L Robertson

January 2023

Membrane proteins are often observed as higher-order oligomers, and in some cases in multiple stoich...

Visualizing single molecular complexes in vivo using advanced fluorescence microscopy.

Dobbie, IM
Robson, A
Delalez, N
Leake, MC

January 2009

Full insight into the mechanisms of living cells can be achieved only by investigating the key proce...

Probing cellular protein complexes using single-molecule pull-down

Jain, Ankur

December 2013

Cellular processes result from dynamic interactions between biomolecules. The gold standard method f...

Measuring the stoichiometry of functional PspA complexes in living bacterial cells by single molecule photobleaching

Lenn, Tchern
Gkekas, Christos N.
Bernard, Laurent
Engl, Christoph
Jovanović, Goran
Buck, Martin
Ying, Liming

January 2011

We report a general method based on wide-field fluorescence imaging of single molecule photobleachin...

The development of a single molecule fluorescence standard and its application in estimating the stoichiometry of the nuclear pore complex

Tie, Hieng Chiong
Madugula, Viswanadh
Lu, Lei

January 2016

We report here an image-based method to quantify the stoichiometry of diffraction-limited sub-cellul...

The development of a single molecule fluorescence standard and its application in estimating the stoichiometry of the nuclear pore complex

Tie, Hieng Chiong
Madugula, Viswanadh
Lu, Lei

September 2016

AbstractWe report here an image-based method to quantify the stoichiometry of diffraction-limited su...

Stoichiometry of molecular complexes at adhesions in living cells.

Digman, Michelle A
Wiseman, Paul W
Choi, Colin
Horwitz, Alan R
Gratton, Enrico

February 2009

We describe a method to detect molecular complexes and measure their stoichiometry in living cells f...

Quantitative Brightness Analysis of Fluorescence Intensity Fluctuations in E. Coli.

Kwang-Ho Hur
Joachim D Mueller

The brightness measured by fluorescence fluctuation spectroscopy specifies the average stoichiometry...

Exploring fluorescence antibunching in solution to determine the stoichiometry of molecular complexes

Sykora, J.
Kaiser, K.
Gregor, I.
Bönigk, W.
Schmalzing, G.
Enderlein, J.

January 2007

Fluorescence antibunching is a well-known technique for determining the number of independent emitte...

Quantitative Brightness Analysis of Fluorescence Intensity Fluctuations in E. Coli

Kwang-Ho Hur (562936)
Joachim D. Mueller (562940)

June 2015

<div><p>The brightness measured by fluorescence fluctuation spectroscopy specifies the average stoic...

Single Cell GFP-Trap Reveals Stoichiometry and Dynamics of Cytosolic Protein Complexes

Tim Wedeking (1675264)
Sara Löchte (1675258)
Christian P. Richter (1675267)
Maniraj Bhagawati (1675270)
Jacob Piehler (348230)
Changjiang You (1675261)

May 2015

We developed in situ single cell pull-down (SiCPull) of GFP-tagged protein complexes based on microp...

Fluorescence resonance energy transfer-based stoichiometry in living cells

Kenneth Christensen
Joel A. Swanson

January 2002

ABSTRACT Imaging of fluorescence resonance energy transfer (FRET) between fluorescently labeled mole...

A general approach to break the concentration barrier in single-molecule imaging

Loveland, Anna B.
Habuchi, Satoshi
Walter, Johannes C.
Oijen, Antoine M. van

January 2012

Single-molecule fluorescence imaging is often incompatible with physiological protein concentrations...

Protocol for single-molecule fluorescence recovery after photobleaching microscopy to analyze the dynamics and spatial locations of nuclear transmembrane proteins in live cells

Mark Tingey
Yichen Li
Weidong Yang

June 2021

Summary: Single-molecule fluorescence recovery after photobleaching (smFRAP) is a newly developed te...

Single-molecule fluorescence of internalised biomolecules in live bacteria

Plochowietz, Anne
Kapanidis, Achillefs N

January 2012

In the last two decades emerging single-molecule fluorescence tools have been developed and adapted ...

A single-molecule method for measuring fluorophore labeling yields for the study of membrane protein oligomerization in membranes.

Melanie Ernst
Tugba N Ozturk
Janice L Robertson

January 2023

Membrane proteins are often observed as higher-order oligomers, and in some cases in multiple stoich...

Visualizing single molecular complexes in vivo using advanced fluorescence microscopy.

Dobbie, IM
Robson, A
Delalez, N
Leake, MC

January 2009

Full insight into the mechanisms of living cells can be achieved only by investigating the key proce...

Probing cellular protein complexes using single-molecule pull-down

Jain, Ankur

December 2013

Cellular processes result from dynamic interactions between biomolecules. The gold standard method f...

Measuring the stoichiometry of functional PspA complexes in living bacterial cells by single molecule photobleaching

Abstract

Extracted data

Measuring the stoichiometry of functional PspA complexes in living bacterial cells by single molecule photobleaching

Abstract

Extracted data

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