Diaph2 expression in E17.5 and E18.5 wild-type mouse cochlea.

Brown indicates positive staining. (A-A”) Vibratome cross section of a E17.5 wild-type mouse cochlea showing Diaph2 in green (A) and the hair-cell marker Myosin VI in red (A’). The merged image of the red and green channels shows the co-localization of Diaph2 with the marker in OHCs. (B-B”‘) Cross section of the cochlear turns (B’: apical, B, B”‘: mid, B”: basal turn) of a E18.5 wild-type mouse fetus. At this stage, a different degree of cell differentiation can be observed from the base to the apex. The square brackets indicate OHCs and the arrowheads indicate Kölliker’s organ. Dorsal to the bottom. Scale bars 10 μm.