Time-dependent release of histone H3 in heparin treated and untreated kidneys with warm ischemic injury.
- Tim C. van Smaalen (5728469)
- Danielle M. H. Beurskens (14485618)
- Jasper J. H. F. M. Kox (14485621)
- Rasheendra Polonia (14485624)
- Rein Vos (582354)
- Hans Duimel (325720)
- Willine J. van de Wetering (14485627)
- Carmen López-Iglesias (3080472)
- Chris P. Reutelingsperger (14485630)
- L. W. Ernest van Heurn (12895085)
- Carine J. Peutz-Kootstra (14485633)
- Gerry A. F. Nicolaes (11416394)
Publication date
January 2023
DOI Publisher
Public Library of Science (PLoS)Abstract
Extracellular histone H3 was determined in kidneys with or without circulating nonanticoagulant heparin. Kidneys treated with heparin showed a similar release in total histone H3 (A) but a more rapid and pronounced increase in levels of a lower molecular weight histone H3 (LMW-H3) variant (B) compared to untreated counter kidneys. (C) A representative Western blot image of full-length histone H3 (17 kDa apparent MW) and LMW-H3 (15 kDa apparent MW) in perfusate of untreated SNMP kidney at t = 5, 6, 7 and 8 hours of perfusion. *Indicates significant differences between groups (p<0.05).</p
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