The endogenous NLRP6 expression is not detected in human colon cancer cell lines after several treatments by western-blotting using an antibody from ABGENT.

(A) Western-blot analysis of extracts of HCT116 or SW480 cells treated during 5hrs with 25μM ALLN or 10μM Lactacystin. (B) Western-blot analysis of extracts of HCT116, RKO or SW480 treated by recombinant human IFN-β at 1ng/mL during 24hrs, recombinant human IFN-λ2 at 2ng/mL during 24hrs, Poly(I:C) LMW at 10μg/mL during 24hrs, or with 20% of culture medium conditioned by NHDFs during the exponential growth phase (CM ExpG NHDFs) or at the senescence plateau (CM Sen NHDFs) during 48hrs. The migration position of NLRP6 is indicated with a dark arrowhead. The white arrowhead points a non-specific doublet at 90kDa. (TIF)