The expression of the 90kDa doublet protein is inducible.

(A) Western-blot analysis of extracts of NHEKs and NHDFs during the exponential growth phase (ExpG) or at the senescence plateau (Sen), and of HCT116, RKO and SW480 cells. (B) Western-blot analysis of extracts of HCT116 and RKO cells treated with recombinant human IFN-γ at 1ng/mL during 24hrs or Poly(I:C). The expected position of NLRP6 is indicated with a dark arrowhead. The white arrowhead points a non-specific doublet at 90kDa whose expression varies in these different conditions. (TIF)