Specificity of a few anti-NLRP6 commercial antibodies.

(A) Schematic representation of the structure of the NLRP6 protein with the position of the epitopes against which the antibodies used in B are directed. (B) Western-blot analysis of extracts of HCT116 cells transiently transfected by the empty or FLAG-NLRP6-expressing pCDNA3.1+ vectors and of non-transfected RKO cells with antibodies from Abiocode and ABGENT. NLRP6 is indicated with a dark arrowhead. The white arrowhead points the non-specific doublet at 90kDa. The red star underlines another non-specific band migrating very close to NLRP6. (C) Western-blot analysis with antibodies from Millipore and ABclonal of extracts of non-transfected HPCEC cells, non-transfected RKO cells and HCT116 cells transiently transfected by the FLAG-NLRP6-expressing pCDNA3.1+ vectors and then transfected with siRNAs against NLRP6 or control siRNAs of non-transfected RKO cells. NLRP6 is indicated with a dark arrowhead. The white arrowhead points the non-specific doublet at 90kDa. (TIF)