The effect of ligand oligomer size and subcellular localization on cis inhibition of Notch signalling.

(A-C) The response of Notch to intracellular ligands of different oligomer size in a cell exposed to fixed levels of trans-ligand (A), in two identical cells (B), and in a single cell (C). Notch activity is normalized against the maximum Notch activity. (D-F) The response of Notch to the production rate of intracellular ligand. Here, we compared simulations of cis inhibition which can occur in all cell compartments (spatially homogeneous), with cis-inhibition occurring only in the cytoplasm.