Infrared (IR) laser ablation was used to remove material from tissue sections mounted on microscope slides, with subsequent capture in a solvent-containing microcentrifuge tube. Experiments conducted with a 3200-bp double-stranded plasmid DNA template demonstrated IR-laser ablation transfer of intact DNA. The transfer efficiency and the molecular integrity of the captured DNA were evaluated using Sanger sequencing, gel electrophoresis, and fluorimetric analysis. The plasmid DNA was reproducibly transferred with an efficiency of 59 ± 3% at laser fluences of between 10 and 20 kJ/m at a wavelength of 3 μm. IR laser ablation sample transfer was then used to ablate and capture DNA from 50-μm-thick rat brain and kidney tissue sections. DNA was ex...
© 2015 American Society for Mass Spectrometry. We have developed a laser ablation sampling technique...
Background Laser capture microdissection (LCM) can be applied to tissues where cells of interest are...
This thesis focuses on the use of laser capture microdissection (LCM) in genomic applications. It ai...
RNA was obtained from discrete locations of frozen rat brain tissue sections through infrared (IR) l...
© 2019 Elsevier B.V. Tip-enhanced laser ablation was used to extract DNA plasmid for polymerase cha...
A high precision infrared laser ablation sampling system was developed for localized tissue proteomi...
An infrared laser was used to ablate material from tissue sections under ambient conditions for dire...
© 2016 John Wiley & Sons, Ltd. We have developed a mid-infrared laser ablation sampling technique fo...
AbstractLaser capture microdissection (LCM) is a well-established cell separation technique. It comb...
Laser capture microdissection (LCM) is a well-established cell separation technique. It combines mic...
© Springer Science+Business Media New York 2015. All rights are reserved. Infrared laser ablation sa...
We have demonstrated an on-line laser ablation sampling system and coupling of the system to liquid ...
New sampling and ionization techniques were developed using a deep-ultraviolet (DUV) laser at 193 nm...
The investigation of in vivo DNA repair in mammalian cells at nucleotide resolution requires the qua...
Abstract Background Laser capture microdissection (LCM) can be applied to tissues where cells of int...
© 2015 American Society for Mass Spectrometry. We have developed a laser ablation sampling technique...
Background Laser capture microdissection (LCM) can be applied to tissues where cells of interest are...
This thesis focuses on the use of laser capture microdissection (LCM) in genomic applications. It ai...
RNA was obtained from discrete locations of frozen rat brain tissue sections through infrared (IR) l...
© 2019 Elsevier B.V. Tip-enhanced laser ablation was used to extract DNA plasmid for polymerase cha...
A high precision infrared laser ablation sampling system was developed for localized tissue proteomi...
An infrared laser was used to ablate material from tissue sections under ambient conditions for dire...
© 2016 John Wiley & Sons, Ltd. We have developed a mid-infrared laser ablation sampling technique fo...
AbstractLaser capture microdissection (LCM) is a well-established cell separation technique. It comb...
Laser capture microdissection (LCM) is a well-established cell separation technique. It combines mic...
© Springer Science+Business Media New York 2015. All rights are reserved. Infrared laser ablation sa...
We have demonstrated an on-line laser ablation sampling system and coupling of the system to liquid ...
New sampling and ionization techniques were developed using a deep-ultraviolet (DUV) laser at 193 nm...
The investigation of in vivo DNA repair in mammalian cells at nucleotide resolution requires the qua...
Abstract Background Laser capture microdissection (LCM) can be applied to tissues where cells of int...
© 2015 American Society for Mass Spectrometry. We have developed a laser ablation sampling technique...
Background Laser capture microdissection (LCM) can be applied to tissues where cells of interest are...
This thesis focuses on the use of laser capture microdissection (LCM) in genomic applications. It ai...