Anthocyanidins promotes beiging of white adipose tissue in mice fed a high fat diet

Supplementation with anthocyanidins (AC), i.e. cyanidin and delphinidin,caused a decrease in body weight gain and fat deposits in mice fed ahigh fat diet (HF). This study investigated the capacity of dietary AC topromote ?beiging? of subcutaneous white adipose tissue (sWAT),through increased mitochondrial biogenesis and thermogenesis. C57BL/6J male mice were fed control (C) or HF diets, with or without supplementationwith 40 mg AC/kg body weight (CA and HFA). After 15 w, HFconsumption caused obesity and increased eWAT weight, which weremitigated by AC supplementation. Adipocyte diameter was higher in HFthan in C, CA and HFA mice. H&E stain showed images compatible withbeiging in both AC-treated groups. Electron microscopy (TEM) showed afewer number of mitochondria in HF but not in HFA mice. TEM findingswere confirmed by measuring the expression of mitochondrial proteinmarkers. AC prevented HF-induced inhibition of the pathway leading tomitochondria biogenesis (PPARγ, PRDM16, PGC-1α), and thermogenicrespiration (UCP-1). Mice consuming the control diet plus AC alsoshowed upregulation of PPARγ, PRDM16, PGC-1α. Findings suggest thatconsumption of selct AC could be an important strategy to mitigate HFinducedobesity in part via activation of adipocyte mitochondriogenesisand beiging.Fil: Rodriguez Lanzi, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Cremonini, Eleonora. University of California. Department of Nutrition and Department of Environmental Toxicology; Estados UnidosFil: Marino, Mirko. University of California. Department of Nutrition and Department of Environmental Toxicology; Estados UnidosFil: Iglesias, Dario Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; ArgentinaFil: Mastaloudis, Angela. No especifíca;Fil: Wood, Steve. No especifíca;Fil: Hester, Shelly. No especifíca;Fil: Fraga, César Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analitica y Fisicoquímica. Cátedra de Fisicoquímica; ArgentinaFil: Oteiza, Patricia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaAnnual Meeting Abstracts “Redox Homeostasis: From Signaling to Damage”FerraraItaliaSociety for Free Radical Research Europ