Spatially controlled staining and collection of 4T1 tumor cells using the HFSM.

A A single fluorescence image of 4T1 cells visualized after staining by three different DiI stream paths (flow rate ratios of 5:2:35, 20:2:20, and 35:2:5 (μL/min)). B High magnification image of 4T1 cells stained with DiI at a flow rate ratio of 20:2:20 (μL/min). C, D Brightfield images of 4T1 cells in the culture chamber (C) before and (D) after exposure to trypsin EDTA flow. Black dotted lines present the stream path of trypsin EDTA. E Brightfield image of collected 4T1 cells after detachment from the chamber by exposure to trypsin EDTA flow.