The LHX2 and OTX2 co-factors in human RPE.

(A) Table summarizing the statistical analysis (t test, two-tailed, paired) and the Log2 fold change in proteins bound by LHX2, OTX2, and BRG1 compared to proteins bound by nonspecific IgG based on immunoprecipitation followed by mass spectrometry (IP-MS) analyses. (B) 293T cells overexpressing OTX2, LDB1, LHX2 in the indicated combinations (Input), were subjected to IP with Flag antibodies followed by western blot analyses and immunolabeling with OTX2 antibody, the arrow points to OTX2. S1 Raw image.). (C) Illustration (using Biorander) of the identified co-factors and key downstream targets in hES-RPE. IP, immunoprecipitation; Mitf, microphthalmia-associated TF; RPE, retinal pigmented epithelium; TF, transcription factor; TSS, transcription start site.