Effect of ustekinumab and identification of human IgG positive cells in the grafts two weeks post-engraftment.

Protein levels of human IL-17 A (a), IL-22 (b), IFN-γ (c), TNF-α (d) and IL-23 (e) were analysed in keratome (Five psoriasis vulgaris patients were included in the study and three biopsies were obtained per keratome for MSD analyses, n = 15) and graft lysates from hIL-2 NOG mice treated with either ustekinumab or isotype control one week after the final dose. The number of mice were n = 14 (isotype) and n = 15 (ustekinumab). (f) Exposure of ustekinumab was evaluated by analyzing human IgG against an ustekinumab standard curve in serum from ustekinumab treated mice (n = 15) and a control group (n = 13). (g) Immunohistochemical staining with HRP conjugated rabbit anti-human IgG of keratomes pre-engraftment (n = 15) and grafts from controls treated with rat IgG2b isotype (n = 13) and ustekinumab treated mice (n = 15). The HRP conjugated rabbit anti-human IgG did not cross-react to rat. Representative slides are shown. The bar equals to 50 μm and slides are shown in a 20X magnification. LLOD is lower limit of detection and HLOD is higher limit of detection.