ARMH4 and WIPF3 in the healthy and disease state.

A: the RNA level of ARMH4 is reduced in patients suffering from collapsing (coll.) and classic (class.) FSGS compared to normal kidney (NK), but not in those diagnosed with minimal change disease (MCD). The WIPF3 level, on the other hand, was not significantly different in any of the diseases (B). C: immunohistochemistry of glomeruli in patients diagnosed with MCD (n = 2), FSGS (n = 2), MN (n = 2) and CG (n = 2) suggests lower intensity of ARMH4 immunoreactivity vs. normal kidney (NK). In CG, ARMH4 is not seen in the crescent of a glomerulus while some staining is maintained in the healthy-looking part of the same glomerulus (G1 = crescent vs. G2 = healthy tissue). The glomerular and tubular morphology were supported by H&E staining (CG-H&E). Arrows in CG show ARMH4 in some cells of a swollen proximal tubule. Scale bars: 100 μm.