Protective effect of MSI-1436 on Tunicamycin-induced ER-stress in HepG2 cells.

(A) Representative bar charts of the relative gene expression of ER-stress markers. (B) Histogram showing the relative expression of spliced XBP1 in comparison to the full-length expression. (C) The gel electrophoresis pattern of the spliced and unspliced amplicons as studied by RT-PCR. The results are expressed as the mean of 3 different experiments ± SD. Asterisks refer to a significant difference between two groups, where: p < 0.05 (*); p < 0.01(**); p < 0.001(***); p < 0.0001 (****). HE: Healthy untreated HepG2 cells; ERS: Endoplasmic reticulum-stressed cells; ERS+MSI: Groups pretreated with MSI-1436 compound before inducing ERS.