The experimental diagram for two-way choice feeding assay.

(a) The preference for 0.25% agarose after dyes were switched. 5 mM sucrose was added to both 0.25% and 0.4% agarose-contained group. Dyes were switched and w1118 males were tested. n = 9 for both group; mean ± SEM. (b) The preference for food hardness of w1118 males in the two-way choice feeding assay. PI for 0.25% of w1118 males tested under 5 concentrations of stiffness (0.25% vs 0.25% ~ 0.5%). 5 mM sucrose was added to different concentrations of agarose. Each gray point represents one independent trial and the number of points per bar indicates the number of replications in each experiment. n = 10, 8, 10, 8 and 8 for each group; mean ± SEM. (TIF)