<i>Fzr</i> mutation abrogates PSG growth during silkworm development.

(A) Transgenic Cas9 protein was specifically expressed in PSG cells. (B-E) The size of PSG from silkworm larvae at L2 (B), L3 (C), L4D2 (D), and L5D3 (E). L2, the second larval instar; L3, the third larval instar; L4D2, the second day of the fourth larval instar; L5D3, the third day of the fifth larval instar. Scale bar for L2 and L3, 2 mm; Scale bar for L4D2 and L5D3, 1 cm. (TIF)