Expression profile of cell cycle-related genes in the PSG during silkworm development.

(A) Fzr was continuously expressed in the PSG during the fourth larval instar, while CycB was not expressed. The Actin gene was used as the internal control. L4D0, just the fourth larval instar; L4D1, the first day of the fourth larval instar; L4D2, the second day of the fourth larval instar; L4D3, the third day of the fourth larval instar. (B) PSG-specific Fzr mutation promoted CycB transcription. (C-E) Fzr mutation decreased the ubiquitinated level of H2B protein (C), Myc transcription (D), and the transcription of the MCM genes (E) in the PSG. Values were represented as means ±S.E. (error bars). For the significance test: *P P P (TIF)