<i>Fzr</i> mutation blocks endoreplication in PSG cells.

(A) EdU staining in silkworm PSG cells. The PSG cells of the control could be strongly stained with EdU, but no EdU signal was detected in PSG cells with Fzr mutation. (B-C) PSG-specific Fzr mutation decreased C value (B) and cell size (C). (D-E) RT-qPCR examination and western blotting analysis for the effects of PSG-specific Fzr mutation on the expression of cell cycle-related factors. Values were represented as means ±S.E. (error bars). For the significance test: *P P P < 0.001 versus the control. Scale bar, 100 μm. WT, wild type.