Fzr regulation on protein synthesis and degradation.

(A) All DEPs in Ribosome pathway were downregulated in PSG cells following PSG-specific Fzr mutation. (B-C) DEPs in the ubiquitin-mediated proteolysis pathway (B) and lysosome pathway (C) were downregulated by Fzr mutation in the PSG. (D) Western blotting showed that RpS15A and RpS25 were downregulated, but GNS and UBE2C were elevated following Fzr mutation. (E) Translating ribosome affinity purification (TRAP) analysis showed that Fzr mutation inhibited mRNA translation in PSG cells. (F) Fzr mutation elevated protein ubiquitination in PSG cells. Values were represented as means ±S.E. (error bars). For the significance test: *P P P < 0.001 versus the control. WT, wild type.