Porovnání promotorů pro využití v klíštěcím (\kur{Ixodes}) expresním systému

Gene manipulation can be a convenient tool for tick control, however functional procedure for gene manipulation of ticks was not determined yet. The creation of plasmid vectors with high ability of gene expression driven by functional promoters is crucial for genetically modified ticks. To investigate effective tools for tick genes manipulation within the scope of this master thesis, transfection procedures for various types of Ixodes tick cell lines were optimized. As well as expression vectors for use in tick cells were tested. For this purpose, expression plasmid vectors containing luciferase reporter genes driven by eukaryotic and viral promoters were used