Silver nanoparticle-protein interactions and the role of lysozyme as an antagonistic antibacterial agent
- Espeche Turbay, Maria Beatriz
- Rey, Valentina
- Dorado, Rita Daniela
- Sosa Morales, Marcelo Clemente
- Borsarelli, Claudio Darío
DOIAbstract
The photoreductive synthesis and antibacterial activity of silver nanoparticles (AgNP) prepared in the presence of bovine serum albumin (BSA) and lysozyme (LZ) were evaluated. AgNP@BSA showed similar antibacterial activity to those stabilized with citrate (AgNP@CIT) and to an AgNO3 solution, suggesting the releases of Ag+ as the mechanism of death. In contrast, AgNP@LZ solutions showed no activity, although LZ behaves as a moderately antibacterial peptide. Furthermore, the addition of LZ to the AgNP@CIT or AgNP@BSA solutions induced their agglomeration and suppressed their original antibacterial efficacy. This antagonistic antibacterial effect exerted by LZ on AgNPs is associated with electrostatic interactions exerted by LZ. Specific metal...