Techniques of Using Peripheral Blood Mononuclear Cells as the Cellular System to Investigate How of the Bovine Species (Indian Zebu-Jersey Crossbreds) Responds to <em>in vitro</em> Thermal Stress Stimulation (Thermal Assault/Heat Shock)

Animal production is negatively impacted by global warming and is subject to serious consequences for livestock production systems. In order to understand how PBMCs of Indian Zebu-Jersey crossbreds respond to various levels and durations of thermal assault and heat shock, in this chapter we will discuss techniques involving in vitro thermal stress stimulation (TSS) to stimulate bovine peripheral blood mononuclear cells (PBMCs) under various thermal assault conditions (TACs), including normal to extreme temperatures and varying durations of thermal exposure (DTEs). The consequences of thermal stress on bovine species can be lessened and managed with an understanding of how PBMCs as a cellular system respond to in vitro heat shock and thermal assault. To learn more about how Indian Zebu-Jersey crossbreds respond to in vitro thermal conditions, it may also be possible to explore the relationship between the decrease in PBMCs count during in vitro TSS and the expression of the heat shock protein genes (HSPs) such as HSPs 70 and 90 genes. This will be exploited to discover how Indian Zebu-Jersey crossbreds respond in vivo to diverse environmental thermal conditions and will further enable in vivo understanding of the potential for thermotolerance in bovine species for better adaptability, survival, and production performance