STEADY-STATE AND DECAY CHARACTERISTICS OF PROTEIN TRYPTOPHAN FLUORESCENCE FROM BACTERIA.
- Dalterio, R. A.
- Nelson, W. H.
- Britt, D.
- Sperry, J.
- Psaras, D.
- Tanguay, J. F.
- Suib, S. L.
DOIAbstract
The intrinsic steady-state fluorescence and fluorescence decay of Staphylococcus epidermidis, Pseudomonas fluorescens, Enterobacter cloacae, Escherichia coli, and Bacillus subtilis have been observed. Each organism exhibits a strong maximum in its emission spectrum at 330-340 nm when excited at 290 nm. Iodide quenching and denaturization experiments with 8 M urea provide strong evidence for the assignment of the 330-340-nm fluorescence to protein tryptophan. Most importantly, the decay of this bacterial protein-tryptophan fluorescence has been described by two exponential functions in all cases
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