Residual nitrogen from slurry and mineral fertiliser two years after application : Fractionation and plant availability

Fertilisation with animal manure has a long-standing tradition as a way to close nutrient cycles on farms. However, the fate of nitrogen (N) from manure in the soil beyond the year of application remains poorly understood. The aim of this research was to understand the residual N fertiliser value of mineral fertiliser (Min) and cattle slurry (Slu) in relation to the partitioning of N from the fertilisers in different soil physical fractions. To this end, we characterised the fate of 15N-labelled Min and Slu in soil organic matter (SOM) physical fractions two years after field application and related it to plant uptake of residual N. A secondary objective was to compare two fractionation methods with respect to accuracy and easiness, namely a combined density and particle size fractionation, separating five fractions, and a simple particle size fractionation, separating two fractions. All fractions were analysed for 15N abundance. The residual N fertiliser value was determined as the uptake of 15N by ryegrass (Lolium multiflorum) during 6 weeks. Furthermore, we deduced the source of 15N taken up by the plants from changes in the percentage of N derived from labelled fertilisers in each of the SOM physical fractions obtained by simple size fractionation before and after the pot experiment. Two years after application, most 15N was found in the fractions