Tunable Preparation of SERS-Active Au–Ag Janus@Au NPs for Label-Free Staphylococcal Enterotoxin C Detection

Trace staphylococcal enterotoxin C (SEC) in food poses a serious risk to human health, and it is vital to develop a sensitive and accurate approach for SEC monitoring. Herein, a surface-enhanced Raman scattering (SERS) aptasensor was developed for the quantitative detection of SEC. SERS-active gold–silver Janus@gold nanoparticles (Au–Ag Janus@Au NPs) were prepared and showed tunable solid and hollow nanostructures by simply controlling the pH values of the reaction system. Solid Au–Ag Janus@Au NPs exhibited intrinsic and enhanced SERS activity due to the intense plasmonic coupling effect between Au dots and Au–Ag Janus NPs, which was 2.27-fold and 17.46-fold higher than that of Au–Ag Janus NPs and hollow Au–Ag Janus@Au NPs, respectively. The attachment of multiple Au dots also protected Ag islands from oxidization, which increased the stability of Au–Ag Janus@Au NPs. Solid Au–Ag Janus@Au NPs served as a label-free, strong, and stable SERS detection probe and achieved sensitive and reliable detection of SEC. The limit of detection was as low as 0.55 pg/mL. This study will expand the application prospects of label-free SERS detection probes in complex systems for food safety monitoring