Miniaturisation of metabolomic and fluxomic workflow on spheroids cultures applied to the treatment of gastric cancer.

International audienceSpheroid (3D) culture models are becoming essential to mimic what happens in tissues in vivo. They are more representative of the cell-cellcontact, dynamic interactions and reduce the impact of plastic compared to traditional 2D cultures. According to the models of cultures,metabolic differences were observed (in glycolytic activity, ATP production and sugar nucleotides synthesis). Expression of drug resistance genesmay be also different and spheroids are often more resistant to the drug than 2D cultures. Culture of single spheroids are used to analyse withhigh throughput a large number of conditions. The challenge of this project was to be able to perform a study of metabolism for single spheroid(metabolomics and 13C metabolomics) of gastric cancer cell line