Abstract Systematic quantification of phosphoprotein within cell signaling networks in solid tissues remains challenging and precise quantification in large scale samples has great potential for biomarker identification and validation. We developed a reverse phase protein array (RPPA) based phosphor-antibody characterization approach by taking advantage of the lysis buffer compatible with alkaline phosphatase (AP) treatment that differs from the conventional RPPA antibody validation procedure and applied it onto fresh frozen (FF) and formalin-fixed and paraffin-embedded tissue (FFPE) to test its applicability. By screening 106 phospho-antibodies using RPPA, we demonstrated that AP treatment could serve as an independent factor to be adopted...
Chad J Creighton,1,3 Shixia Huang2,3 1Department of Medicine, 2Department of Molecular and Cellular...
AbstractDefining alterations in signalling pathways in normal and malignant cells is becoming a majo...
Defining alterations in signalling pathways in normal and malignant cells is becoming a major field ...
Reverse Phase Protein Arrays (RPPA) represent a very promising sensitive and precise high-throughput...
Mastering the systematic analysis of tumor tissues on a large scale has long been a technical challe...
Factors including intratumoral heterogeneity and variability in tissue handling potentially hamper t...
Reverse Phase Protein Array (RPPA) represents a sensitive and high-throughput technique allowing sim...
Reverse phase protein arrays (RPPA) can assess protein expression and activation states in large num...
<p>As an example to illustrate the concept of RPPA, the results of four arrays probed with antibodie...
Precise quantitation of protein biomarkers in clinical tissue specimens is a prerequisite for accura...
<p>For the correction of anti-species secondary antibody staining, arrays were assayed in the absenc...
Protein phosphorylation is one of the most common and important post-translational modifications and...
'Reverse-phase' protein lysate microarray (RPA) assays use micro-scale, cell lysate dot blots that a...
<div><p>Precise quantitation of protein biomarkers in clinical tissue specimens is a prerequisite fo...
Abstract An encouraging approach for the diagnosis and effective therapy of immunological pathologie...
Chad J Creighton,1,3 Shixia Huang2,3 1Department of Medicine, 2Department of Molecular and Cellular...
AbstractDefining alterations in signalling pathways in normal and malignant cells is becoming a majo...
Defining alterations in signalling pathways in normal and malignant cells is becoming a major field ...
Reverse Phase Protein Arrays (RPPA) represent a very promising sensitive and precise high-throughput...
Mastering the systematic analysis of tumor tissues on a large scale has long been a technical challe...
Factors including intratumoral heterogeneity and variability in tissue handling potentially hamper t...
Reverse Phase Protein Array (RPPA) represents a sensitive and high-throughput technique allowing sim...
Reverse phase protein arrays (RPPA) can assess protein expression and activation states in large num...
<p>As an example to illustrate the concept of RPPA, the results of four arrays probed with antibodie...
Precise quantitation of protein biomarkers in clinical tissue specimens is a prerequisite for accura...
<p>For the correction of anti-species secondary antibody staining, arrays were assayed in the absenc...
Protein phosphorylation is one of the most common and important post-translational modifications and...
'Reverse-phase' protein lysate microarray (RPA) assays use micro-scale, cell lysate dot blots that a...
<div><p>Precise quantitation of protein biomarkers in clinical tissue specimens is a prerequisite fo...
Abstract An encouraging approach for the diagnosis and effective therapy of immunological pathologie...
Chad J Creighton,1,3 Shixia Huang2,3 1Department of Medicine, 2Department of Molecular and Cellular...
AbstractDefining alterations in signalling pathways in normal and malignant cells is becoming a majo...
Defining alterations in signalling pathways in normal and malignant cells is becoming a major field ...