A large number of fish are prone to death during the passage through pumping stations according to the monitoring data, especially when the axial-flow pumps are equipped. This study comprises the assessment of fish damage for traditional axial-flow pump and the redesigned so-call fish-friendly pump. The Lagrangian tracking approach combined with computational fluid dynamics that takes the fish profile and contact force into account is used to shed light on the fish motion behaviors. The three most important mechanisms for fish injury (i.e. the strike of blade leading edge, pressure reduction and shear force) are discussed. Results show that the fish-friendly pump with reduced number of blades leads to sharp drop of strike probability and it...