Secretory response of 10w old islets and α-cell morphometry analysis.

A. Insulin secretory responses to low (5.5mM) and high (12.5mM) glucose of islets from 10w old Nkcc1βKO and control mice (Ins1Cre) in the presence of vehicle (DMSO) or 10μM bumetanide (BTD), as indicated. Results are expressed as the mean ± SEM of insulin secreted relative to total islet insulin content (n = 3, *pB-F. Shown are α-cell number (B, counts per islet), volume (C, pL), area (D, μm2; and E, % of pancreas section) and α-cell mass (F, g) corresponding to 10w and 30w old Nkcc1βKO and control (Nkcc1lox/lox) mice. The results in B-D represent the mean ± SEM of data corresponding to >700 individual glucagon-stained islets found in 19–21 pancreas tissue sections obtained from male mice (n = 3) of the indicated genotypes. Each point in E, F represents mean values per tissue section. G. Shown is the mean islet α/β-cell ratio of Nkcc1βKO and control mice at the indicated ages (*pFig 2B. (TIF)