Antithrombotic activity of flavonoid fractions of Juglans regia root bark through inhibiting platelet aggregation and plasmatic coagulation

Thrombus formation in cardiovascular diseases is one of the important mechanisms responsible for death and disability. Plants have long been used as traditional remedies for the treatment of many diseases.In this study, we investigated the antithrombotic, antiaggregant, and anticoagulant activities of flavonoids isolated from Juglans regia root bark.The antithrombotic activity was evaluated on collagen plus epinephrine induced acute pulmonary thromboembolism model of mice and murine platelet aggregation was induced by different agonists. Clotting parameters including activated partial thromboplastin time, prothrombin time, and thrombin time in the platelet-poor plasma were determined in vitro by using a coagulometer in the presence and absence of flavonoid fractions.Flavonoids extracted from Juglans regia exhibited an antithrombotic activity (80% of protection) by preventing the paralysis or death induced by an intravenous injection of collagen and epinephrine. In addition, these compounds significantly inhibited platelet aggregation and prolonged the plasmatic coagulation time.Flavonoids isolated from Juglans regia exert a potent antithrombotic effect via antiaggregant and anticoagulant actions. Juglans regia could be a precious source of antithrombotic compounds