Periodontal disease related to oxidative stress

Th e study evaluates oxidative stress (OS) in patients with diff erent stages of periodontal disease (PD) and the infl uence of their smoking habits on OS. PD is related to connective tissue infl ammation, which leads to deterioration of the supporting structures of the tooth. OS is a condition characterised by overproduction of free radicals (FR), which may be involved in PD, causing periodontal tissue damage and other related diseases. Study patients' were grouped as I-non smokers (n=45) and II-smokers (n=45); and consisted of patients with 3 PD stages: mild (1), moderate (2) and severe (3). As a control group, 30 healthy subjects (all non smokers) with no signs of PD were selected. FR levels were determined by the D-Roms test, total antioxidant capacity (AOC) was determined by the OXY-adsorbent test, (Diacron, Italy) and lipid peroxidation (LP) was determined by the fl uorometric method with thiobarbituric acid and its end product, malonyldialdehyde (MDA). OS was found in the periodontal tissue and serum of PD patients, with the highest level of FR in the severe stage (3) in non smokers (p<0.05) as well as smokers (p<0.01); AOC showed decreasing values from mild (1) to severe stage (3) of PD for smokers (p<0.05). LP in serum showed the highest level in severe stage (3) in both groups i.e., non smokers and smokers (p