Thidiazuron-induced formation of strawberry microshoots on different nutrient media

The present study was aimed to improve in vitro shoot proliferation of octoploid strawberry cultivar ‘Solnechnaya polyanka’ on different nutrient media (MS, B5 or MS+B5), supplemented with various concentrations (2.0, 4.0, 8.0 ȝM) of thidiazuron (TDZ). The best regeneration frequency (100%) with the highest rate of strawberry axillary shoot proliferation (14.1 ± 1.24 shoots per explant) and maximum shoot length (1.92 ± 0.04 cm) were achieved using precultivation of explants for 3 days on induction media B5 supplemented with 4.0 ȝM TDZ followed by cultivation of regenerants on hormone-free media B5 for 6 weeks