Effects of temperature on the virulence of <i>M</i>. <i>abscessus</i> subsp. <i>abscessus</i> ATCC19977 in silkworms.

Silkworms were injected with saline (50 μl) or M. abscessus subsp. abscessus ATCC19977 cell suspension (1.4 x 107 cells per 50 μl) and incubated at (A) 27˚C and (B) 37˚C. The curves were drawn by the Kaplan-Meier method. Seven silkworms were used per group. (C) Silkworms were injected with M. abscessus subsp. abscessus ATCC19977 cell suspension (2.9 x 108 cells per 50 μl) and incubated at 27˚C and 37˚C. Silkworm hemolymph was harvested at 18 h post-infection. Statistically significant differences between groups were evaluated using the Student t-test. Three silkworms were used per group.