Determination of the <i>M</i>. <i>abscessus</i> subsp. <i>abscessus</i> ATCC19977 LD₅₀ in silkworms.

Silkworms were injected with saline (50 μl) or M. abscessus subsp. abscessus ATCC19977 cell suspension (4 x 105–1 x 108 cells per 50 μl) and incubated at 37˚C for 2 days. The numbers of live and dead silkworms are indicated as 1 and 0, respectively. The curve represents data from 6 independent experiments combined in a simple logistic regression model. One hundred twenty-nine silkworms were used in the experiment.