Effects of autoclaved cells and antibacterial treatment in silkworms infected with <i>M</i>. <i>abscessus</i> subsp. <i>abscessus</i>.

(A) Silkworms were injected with either saline (50 μl), M. abscessus subsp. abscessus ATCC19977 cell suspension (1.1 x 107 cells per 50 μl), or autoclaved M. abscessus subsp. abscessus ATCC19977 cell suspension and incubated at 37˚C. Ten silkworms were used per group. (B) Silkworms were injected with either saline (50 μl) or M. abscessus subsp. abscessus ATCC19977 cell suspension (6.3 x 107 cells per 50 μl) followed by clarithromycin (25 μg g-1 larva). The number of surviving silkworms following incubation at 37˚C was measured for 66 h. Statistically significant differences between groups were evaluated using a log-rank test based on the curves by the Kaplan-Meier method. Ten silkworms were used per group.