Supplemental Figure S1

Control measurements of RyR phosphorylation changes upon β-adrenergic stimulation and inhibition of kinases in mouse atrial cardiomyocytes. A. Representative confocal images of isolated mouse atrial myocyte stained with total RyR (tRyR, green), pRyR-Ser2030 (red) and merge between them. Cells were treated with normal IT buffer (top), isoprenaline (middle) and H-89 and KN93 (bottom). Right panel shows the average levels of % pRyR-Ser2030 in control (n=37), isoprenaline (n=14), and H89 with KN93 (n=16) treated cells. **, *** – pB. Typical confocal images of atrial myocyte stained with tRyR (green), pRyR-Ser2808 (red) and merge between them. Cells were treated same way as panel A. Right panel shows the average levels of pRyR-Ser2808 in control (n=25), isoprenaline (n=8), and H89 with KN93 (n=7) treated cells. *, *** – pC. Representative confocal images of atrial myocytes stained with tRyR (green), pRyR-Ser2814 (red) and merge between them. Cells were treated same way as panel A. Bar graph on the right shows the analysis of pRyR-Ser2814 in control (n=33), isoprenaline (n=21), and H89 with KN93 (n=9) treated cells. ** – p<0.01.